vectastain eliteabc kit (Vector Laboratories)

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Vector Laboratories vectastain eliteabc kit
Vectastain Eliteabc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 16781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+eliteabc+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Universal)/pm39684417-351-8-12
Average 96 stars, based on 16781 article reviews

vectastain eliteabc kit - by Bioz Stars, 2026-09

96/100 stars

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Immunohistochemistry:

Article Title: LMO3 downregulation in PCa: A prospective biomarker associated with immune infiltration
Article Snippet: We obtained patients’ consent and approval from the Institutional Research Ethics Committee, then collected PCa tissues, and matched adjacent normal tissues from Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. .. Then, immunohistochemistry (IHC) staining was performed using the VECTASTAIN EliteABC kit (Vector Laboratories, Burlingame, CA, United States), and its procedures were presented, as previously described ( ). ..

Staining:

Incubation:

Article Title: Bifidobacterium breve Attenuates Murine Dextran Sodium Sulfate-Induced Colitis and Increases Regulatory T Cell Responses
Article Snippet: Slides were washed with PBS and blocked with rabbit or goat serum before an overnight incubation (4°C) with primary antibodies against Ly-6B (AbD Serotec, Dusseldorf, Germany), RORγt (eBioscience San Diego, CA USA) or Foxp3 (eBioscience San Diego, CA USA). .. For detection, biotinylated goat anti-rat (Dako, Glostrup, DK) secondary antibodies were administered followed by incubation with peroxidase-labeled streptavidin (Vectastain EliteABC kit, Vector, Burlingame, CA USA). .. The peroxidase activity was visualized using the substrate, DAB (Sigma, Gillingham, UK).

Article Title: Interleukin-34 produced by human fibroblast-like synovial cells in rheumatoid arthritis supports osteoclastogenesis
Article Snippet: The slides were blocked with 7.5% normal goat serum for 20 minutes and thereafter incubated with 4 μg/ml of IL-34 antibody (LSBio, Seattle, WA, USA) or control rabbit IgG (Abcam, Cambridge, MA, USA) for 20 minutes at room temperature. .. Following two washes with PBS, the slides were incubated with 1:200 diluted biotinylated secondary antibody (Vectastain EliteABC Kit; Vector Laboratories, Orton Southgate, Peterborough, UK) for 30 minutes. .. After washing with PBS, the slides were incubated with complexed avidin-biotin horseradish peroxidase ABC reagent (Vectastain Elite ABCkit; Vector) for 30 minutes, and then exposed to 3,3-diaminobenzidine (DAB) substrate according to the manufacturer's instructions (Vector).

Article Title: Reciprocal interaction between striatal cholinergic and low-threshold spiking interneurons - A computational study.
Article Snippet: .. The slices were thoroughly washed in PBS and incubated in 0.6% hydrogen peroxidase in methanol for 20 min, rinsed in PBS and transferred to the ABC solution (Vectastain EliteABC kit, Vector Laboratories, CA, USA) for 3 hours. .. After rinsing in PBS, the slices containing ChINs were incubated in diaminobenzidine (DAB, ImmPACT DAB, Vector Laboratories, CA, USA) for 3-5 min, rinsed, and dehydrated in alcohol prior to mounting in Entellan (Merck).

Avidin-Biotin Assay:

Article Title: Anorectal Remodeling in the Transitional Zone with Increased Expression of LGR5, SOX9, SOX2, and Keratin 13 and 5 in a Dextran Sodium Sulfate-Induced Mouse Model of Ulcerative Colitis
Article Snippet: .. An avidin-biotin-peroxidase complex method was employed using the VECTASTAIN ® EliteABC Kit (Vector Laboratories Inc., Burlingame, CA, USA) with 3,3′-diaminobenzidine as a chromogen, followed by counterstaining with hematoxylin for microscopic examination. ..

Article Title: Anorectal Remodeling in the Transitional Zone with Increased Expression of LGR5, SOX9, SOX2, and Keratin 13 and 5 in a Dextran Sodium Sulfate-Induced Mouse Model of Ulcerative Colitis.
Article Snippet: .. An avidin-biotin-peroxidase complex method was employed using the VECTASTAIN ® EliteABC Kit (Vector Laboratories Inc., Burlingame, CA, USA) with 3,3′-diaminobenzidine as a chromogen, followed by counterstaining with hematoxylin for microscopic examination. ..

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